AzuraQuant™ II cDNA Synthesis Kit 25 Reactions

The Azura-Quant II cDNA Synthesis Kit has been developed for fast and sensitive first-strand cDNA Synthesis. The kit was developed to provide unbiased synthesis across a wide range of input RNA, up to 2 µg. The AzuraQuant II cDNA Synthesis Kit is supplied with a high-performance 5x cDNA synthesis mix including an optimized ratio of random hexamer primers and anchored oligo (dT) primers, which delivers excellent consistency. The AzuraQuant II cDNA Synthesis Kit delivers both highly efficient first-strand synthesis and higher cDNA yields, leading to enhanced reproducibility and data accuracy. The kit is supplied in a convenient 2-tube format comprised of a 5x cDNA synthesis mix and 20x HighTherm II reverse transcriptase blended with RNase inhibitor. HighTherm II reverse transcriptase exhibits enhanced thermostability and processivity, low RNase H activity, and robust overall performance in cDNA synthesis.

• 2-step RT-qPCR
• 2-step RT-PCR
• Convenience: Two-tube Format and simple 25 minute protocol
• Unbiased, Sensitive cDNA Synthesis: Complete 5' to 3' RNA sequence represenation from 4 pg to 2 µg total RNA.
• Reproducible: HighTherm II reverse transcriptase coupled with an optimized 5x cDNA Buffer system for consistent, high-yield qPCR-ready cDNA.
• Broad Dynamic Range: High-Yield cDNA synthesis across a wide range of RNA concentrations.